a filamentous phage display system for n-linked glycoproteins

Engineering a peptide epitope display system on filamentous.
Strain engineering for improved expression of recombinant proteins.
use of filamentous phage [1], a bacteriophage that lives on Escherichia coli.. The power of the phage display system is illustrated in Fig. 1.. In phagemids, the scFv may be fused at the N-terminus of the mature gene III protein ... triethylamine [22], with DTT when biotin was linked to antigen by a disulphide bridge [20], by.
In this system, we analyzed the interplay between N-linked glycosylation, membrane .. Filamentous fusion phage: novel expression vectors that display cloned.
http://dx.doi.org/10.1016/S0022-1759(97)00083-5, How to Cite or Link Using DOI . mAbGDO5 with the Hantaan virus glycoprotein G2 and mAbL13F3 with the. phage libraries displaying 6mer and 15mer peptides at the N-terminus of the pIII protein. Using the gene-fragment phage display libraries a single round of affinity.

a filamentous phage display system for n-linked glycoproteins

Recombinant Antibodies for Cancer Therapy: Methods and Protocols[ - Google Books Result.
The products of ORFs 2 to 4 are minor membrane-associated glycoproteins (GP2 , GP3. Phage display peptide library technology is a powerful molecular tool.. or proteins on the surface of a filamentous bacteriophage (3, 8, 15, 19, 24). ... the utility of the PRRSV N protein produced in prokaryotic systems (7, 9, 28, 42).

The products of ORFs 2 to 4 are minor membrane-associated glycoproteins (GP2 , GP3. Phage display peptide library technology is a powerful molecular tool.. or proteins on the surface of a filamentous bacteriophage (3, 8, 15, 19, 24). ... the utility of the PRRSV N protein produced in prokaryotic systems (7, 9, 28, 42).

Celik E, Fisher AC, Guarino C, Mansell TJ, Delisa MP: A filamentous phage display system for N-linked glycoproteins. Protein Sci 2010. OpenURL. Durr C.
Dec 2, 2009. Phage display using filamentous phage vectors is a widely used. In most N terminal display systems [p8 (15), p7 (16), p9 (17) and p7 + ... we used two alternative coupling methods to link the exported GFP .. Characterization of BoHV-1 gE envelope glycoprotein mimotopes obtained by phage display.
9:30 A Filamentous Phage Display System for N-linked Glycoproteins. Matthew DeLisa Matthew P. DeLisa, Ph.D., Associate Professor, Chemical & Biomolecular.
Epitope mapping by phage display: Random versus gene-fragment.

Antibody Phage Display Libraries: Contributions to Oncology.

Strain engineering for improved expression of recombinant proteins.

An engineered eukaryotic protein glycosylation pathway in.
Celik, E., Fisher, A., Guarino, C., Mansell, T., and DeLisa, M. (2010). A filamentous phage display system for N-linked glycoproteins. Protein Sci. 19, 2006–2013.
May 4, 2012. Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on filamentous phage surfaces have been used .. system in vitro by specifically enriching phage particles that display antibodies .. fibronectin, a high-molecular weight glycoprotein that binds to integrins [76].